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  2. Biochemical engineering - Wikipedia

    en.wikipedia.org/wiki/Biochemical_engineering

    Bioreactor. Biochemical engineering, also known as bioprocess engineering, is a field of study with roots stemming from chemical engineering and biological engineering.It mainly deals with the design, construction, and advancement of unit processes that involve biological organisms (such as fermentation) or organic molecules (often enzymes) and has various applications in areas of interest ...

  3. Bioprocess - Wikipedia

    en.wikipedia.org/wiki/Bioprocess

    The upstream part of a bioprocess refers to the first step in which microbes/cells are grown, e.g. bacterial or mammalian cell lines (see cell culture), in bioreactors. Upstream processing involves all the steps related to inoculum development, media development, improvement of inoculum by genetic engineering process, optimization of growth ...

  4. Five prime untranslated region - Wikipedia

    en.wikipedia.org/wiki/Five_prime_untranslated_region

    A uORF is a coding sequence located in the 5′ UTR located upstream of the coding sequences initiation site. These uORFs contain their own initiation codon, known as an upstream AUG (uAUG). This codon can be scanned for by ribosomes and then translated to create a product, [ 19 ] which can regulate the translation of the main protein coding ...

  5. Upstream and downstream (DNA) - Wikipedia

    en.wikipedia.org/wiki/Upstream_and_downstream_(DNA)

    Due to the anti-parallel nature of DNA, this means the 3' end of the template strand is upstream of the gene and the 5' end is downstream. Some genes on the same DNA molecule may be transcribed in opposite directions. This means the upstream and downstream areas of the molecule may change depending on which gene is used as the reference.

  6. Untranslated region - Wikipedia

    en.wikipedia.org/wiki/Untranslated_region

    The 5' UTR of prokaryotes consists of the Shine–Dalgarno sequence (5'-AGGAGGU-3'). [6] This sequence is found 3-10 base pairs upstream from the initiation codon. The initiation codon is the start site of translation into protein.

  7. Upstream open reading frame - Wikipedia

    en.wikipedia.org/wiki/Upstream_open_reading_frame

    An upstream open reading frame (uORF) is an open reading frame (ORF) within the 5' untranslated region (5'UTR) of an mRNA. uORFs can regulate eukaryotic gene expression. [1] [2] Translation of the uORF typically inhibits downstream expression of the primary ORF. However, in some genes such as yeast GCN4, translation of specific uORFs may ...

  8. S phase - Wikipedia

    en.wikipedia.org/wiki/S_phase

    This transition is essentially irreversible; after passing the restriction point, the cell will progress through S-phase even if environmental conditions become unfavorable. [2] Accordingly, entry into S-phase is controlled by molecular pathways that facilitate a rapid, unidirectional shift in cell state.

  9. Mung bean nuclease - Wikipedia

    en.wikipedia.org/wiki/Mung_bean_nuclease

    Mung bean nuclease (Nuclease MB) is a nuclease derived from sprouts of the mung bean (Vigna radiata) that removes nucleotides in a step-wise manner from single-stranded DNA molecules (ssDNA) and is used in biotechnological applications to remove such ssDNA from a mixture also containing double-stranded DNA (dsDNA).

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