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Deuterated chloroform is a general purpose NMR solvent, as it is not very chemically reactive and unlikely to exchange its deuterium with its solute, [9] and its low boiling point allows for easy sample recovery. It, however, it is incompatible with strongly basic, nucleophilic, or reducing analytes, including many organometallic compounds.
The dried drop method is the simplest of deposition methods. The matrix and sample solution are mixed together and then a small drop of the mixture is placed on the sample probe surface and allowed to dry, thus crystallizing. The sandwich method involves depositing a layer of matrix onto the surface of the probe and allowing it to dry.
Deuterated chloroform. Deuterated solvents are a group of compounds where one or more hydrogen atoms are substituted by deuterium atoms. These isotopologues of common solvents are often used in nuclear magnetic resonance spectroscopy. [1]
Vacuum drying is the mass transfer operation in which the moisture present in a substance, usually a wet solid, is removed by means of creating a vacuum.. In chemical processing industries like food processing, pharmacology, agriculture, and textiles, drying is an essential unit operation to remove moisture. [1]
Chloroform: organic compound; often used as CDCl3 (deuterated chloroform) as a solvent for NMR spectroscopy and as a general solvent. Chromic acid: a strong and corrosive oxidising agent; an intermediate in chromium plating Chromium trioxide: the acidic anhydride of chromic acid; mainly used in chrome-plating Collins reagent
The chloroform molecule can be viewed as a methane molecule with three hydrogen atoms replaced with three chlorine atoms, leaving a single hydrogen atom. The name "chloroform" is a portmanteau of terchloride (tertiary chloride, a trichloride) and formyle, an obsolete name for the methylylidene radical (CH) derived from formic acid. [citation ...
These solvents tend to be relatively non-polar; they are therefore immiscible with water and effective in cleaning applications such as degreasing and dry cleaning for their ability to dissolve oils and grease. They are mostly nonflammable or have very low flammability.
Thus a molecule that contains some deuterium will be heavier than one that contains all hydrogen. As a protein is increasingly deuterated, the molecular mass increases correspondingly. Detecting the change in the mass of a protein upon deuteration was made possible by modern protein mass spectrometry, first reported in 1991 by Katta and Chait.