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Typical differential centrifugation parameters for a biological sample [2] (path length of centrifugation ≈1–5 cm) Sample input G force Time Instrument needed Pellet contents Supernatant contents Unlysed (eukaryotic) cells 100 x g 5 min Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Intact (eukaryotic) cells, macroscopic debris
Concentration of Plasmodium falciparum-infected erythrocytes by discontinuous density gradient centrifugation in Percoll [1] Percoll is a reagent consisting of colloidal silica particles used in cell biology and other laboratory settings. It was first formulated by Pertoft and colleagues, [2] and commercialized by Pharmacia Fine Chemicals. [3]
Historically a cesium chloride (CsCl) solution was often used, but more commonly used density gradients are sucrose or Percoll.This application requires a solution with high density and yet relatively low viscosity, and CsCl suits it because of its high solubility in water, high density owing to the large mass of Cs, as well as low viscosity and high stability of CsCl solutions.
Differential centrifugation is the simplest method of fractionation by centrifugation, [9] commonly used to separate organelles and membranes found in cells. Organelles generally differ from each other in density and in size, making the use of differential centrifugation, and centrifugation in general, possible.
Laboratory centrifuge. There are various types of centrifugation: Differential centrifugation, often used to separate certain organelles from whole cells for further analysis of specific parts of cells; Isopycnic centrifugation, often used to isolate nucleic acids such as DNA
The term "isopycnic" is also encountered in biophysical chemistry, usually in reference to a process of separating particles, subcellular organelles, or other substances on the basis of their density. Isopycnic centrifugation refers to a method wherein a density gradient is either pre-formed or forms during high speed centrifugation. After this ...
The gas centrifuge consists of a cylindrical rotor, a casing, an electric motor, and three lines for material to travel. The gas centrifuge is designed with a casing that completely encloses the centrifuge. [4] The cylindrical rotor is located inside the casing, which is evacuated of all air to produce a near frictionless rotation when ...
In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component. [1] This is a method that was originally used to demonstrate the cellular location of various biochemical processes.