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Simple Staining is a technique that only uses one type of stain on a slide at a time. Because only one stain is being used, the specimens (for positive stains) or background (for negative stains) will be one color. Therefore, simple stains are typically used for viewing only one organism per slide. Differential staining uses multiple stains per ...
Due to its short staining time, Diff-Quik stain is often used for initial screening of cytopathology specimens. This staining technique allows the cytotechnologist or pathologist to quickly assess the adequacy of the specimen, identify possible neoplastic or inflammatory changes, and decide whether or not additional staining is required. [4] [9 ...
Differential staining is a staining process which uses more than one chemical stain. [1] Using multiple stains can better differentiate between different microorganisms or structures/cellular components of a single organism. Differential staining is used to detect abnormalities in the proportion of different white blood cells in the blood.
Use of sample-staining methods for use in microbiology, such as simple stains (methylene blue, safranin, crystal violet) and differential stains (negative stains, flagellar stains, endospore stains). Use of a colored (usually blue) or polarizing filter on the light source to highlight features not visible under white light.
Differential staining – glacial acetic acid used, no heat applied, secondary stain is Loeffler's methylene blue. Kinyoun modification (or cold Ziehl–Neelsen technique) is also available. A protocol in which a detergent is substituted for the highly toxic phenol in the fuchsin staining solution.
Fungal yeast forms are inconsistently stained with Acid-fast stain which is considered a narrow spectrum stain for fungi. [21] In a study on acid-fastness of fungi, [ 22 ] 60% of blastomyces and 47% of histoplasma showed positive cytoplasmic staining of the yeast-like cells, and Cryptococcus or candida did not stain, and very rare staining was ...
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These stains allow for the detection of white blood cell, red blood cell, and platelet abnormalities. Hematopathologists often use other specialized stains to aid in the differential diagnosis of blood disorders. [citation needed] After staining, the monolayer is viewed under a microscope using magnification up to 1000 times.