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Parfocal microscope objectives stay in focus when magnification is changed; i.e., if the microscope is switched from a lower power objective (e.g., 10×) to a higher power objective (e.g., 40×), the object stays in focus. Most modern bright-field microscopes are parfocal.
The resolution of a microscope is defined as the minimum separation needed between two objects under examination in order for the microscope to discern them as separate objects. This minimum distance is labelled δ. If two objects are separated by a distance shorter than δ, they will appear as a single object in the microscope.
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
Bright-field microscopy is the simplest of a range of techniques used for illumination of samples in light microscopes, and its simplicity makes it a popular technique. The typical appearance of a bright-field microscopy image is a dark sample on a bright background, hence the name.
Cone of light behind an achromatic doublet objective lens (A) without (red) and with (green) a Barlow lens optical element (B). The Barlow lens, named after Peter Barlow, is a diverging lens which, used in series with other optics in an optical system, increases the effective focal length of an optical system as perceived by all components that are after it in the system.
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