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Depending on the size of the pipette, the user needs specific tip sizes like: 10 μL, 100 μL, 200 μL, 1,000 μL, other non-standard sizes, such as 5,000 μL (5 mL) or 10,000 μL (10 mL). The majority of tips have a color code for easy spotting like natural (colorless) for low volumes (0.1–10 μL), yellow (10–100 μL), or blue (100–1,000 ...
These pipettes have a large bulb with a long narrow portion above with a single graduation mark as it is calibrated for a single volume (like a volumetric flask). Typical volumes are 20, 50, and 100 mL. Volumetric pipettes are commonly used to make laboratory solutions from a base stock as well as prepare solutions for titration.
These pipettes commonly come in 5, 10, 25, and 50 mL volumes. A variety of propipetters [clarification needed] have been developed, both entirely manual and electrically assisted. Originally pipettes were made of soda-lime glass, but currently many are made of borosilicate glass; disposable graduated pipettes are often made of polystyrene.
Typical volumes are 1, 2, 5, 10, 20, 25, 50 and 100 mL. Volumetric pipettes are commonly used in analytical chemistry to make laboratory solutions from a base stock as well as to prepare solutions for titration. ASTM standard E969 defines the standard tolerance for volumetric transfer pipettes. The tolerance depends on the size: a 0.5-mL ...
A calibrated dropping pipette, or automatic pipette, delivering drops of 20μl. Petri dishes containing nutrient agar or other appropriate medium. Phosphate Buffered Saline (PBS) or other appropriate diluent. Bacterial suspension or homogenate. Method. The inoculum / suspension is serially diluted by adding 1x of suspension to 9x of diluent.
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