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The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
Phenol red was used by Leonard Rowntree and John Geraghty in the phenolsulfonphthalein test to estimate the overall blood flow through the kidney in 1911. [9] It was the first test of kidney function and was used for almost a century but is now obsolete. The test is based on the fact that phenol red is excreted almost entirely in the urine.
pH indicators: a graphic view. A pH indicator is a halochromic chemical compound added in small amounts to a solution so the pH (acidity or basicity) of the solution can be determined visually or spectroscopically by changes in absorption and/or emission properties. [1]
Reference ranges (reference intervals) for blood tests are sets of values used by a health professional to interpret a set of medical test results from blood samples. Reference ranges for blood tests are studied within the field of clinical chemistry (also known as "clinical biochemistry", "chemical pathology" or "pure blood chemistry"), the ...
Other enterobacteria such as E. coli will ferment the lactose present in the medium to an extent that will prevent pH reversion by decarboxylation and acidify the medium, turning it yellow. Salmonella species: red colonies, some with black centers. The agar itself will turn red due to the presence of Salmonella type colonies. Shigella species ...
The pH indicator Methyl Red is added to one tube and a red color appears at pH's lower than 4.2, indicating a positive test (mixed acid fermentation is used). The solution remaining yellow (pH = 6.2 or above) indicates a negative test, meaning the butanediol fermentation is used. The VP test uses alpha-naphthol and potassium hydroxide to test ...
Lactose fermenters turn red or pink on MacConkey agar, and nonfermenters do not change color. The media inhibits growth of gram-positive organisms with crystal violet and bile salts, allowing for the selection and isolation of gram-negative bacteria. The media detects lactose fermentation by enteric bacteria with the pH indicator neutral red. [2]
Cystine tryptic agar (CTA), also known as cystine trypticase agar, [1] [2] is a growth medium used for the identification of microorganisms. [3]It can be used to determine if organisms can ferment various carbohydrates, including maltose, lactose, and sucrose.