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Tom Maniatis (born May 8, 1943), is an American professor of molecular and cellular biology. He is a professor at Columbia University , and serves as the Scientific Director and CEO of the New York Genome Center .
English: Depiction of one common way to clone a site-directed mutagenesis library (i.e., using degenerate oligos). The gene of interest is PCRed with oligos that contain a region that is perfectly complementary to the template (blue), and one that differs from the template by one or more nucleotides (red).
[3] [4] In 1982, Fritsch, Joe Sambrook, and Maniatis wrote Molecular Cloning: A Laboratory Manual, which was considered "omnipresent in Molecular Biology laboratories and [...] utilized to the point where it is frequently referred to as ‘The Bible’.” [5] Fritsch helped initiate and for four years co-taught the widely successful Cold ...
Recombinant DNA (rDNA), or molecular cloning, is the process by which a single gene, or segment of DNA, is isolated and amplified. Recombinant DNA is also known as in vitro recombination . A cloning vector is a DNA molecule that carries foreign DNA into a host cell , where it replicates, producing many copies of itself along with the foreign DNA.
Molecular cloning takes advantage of the fact that the chemical structure of DNA is fundamentally the same in all living organisms. Therefore, if any segment of DNA from any organism is inserted into a DNA segment containing the molecular sequences required for DNA replication, and the resulting recombinant DNA is introduced into the organism from which the replication sequences were obtained ...
Giannis Maniatis, Greek international football (soccer) player; Kostas Maniatis, professional football (soccer) goalkeeper; Tom Maniatis, professor of molecular and cellular biology; Peter Maniatis, TV host and boxing promoter
Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or proteins in a matrix of agarose, one of the two main components of agar.
Transcription activator-like effector nucleases (TALEN) are restriction enzymes that can be engineered to cut specific sequences of DNA. They are made by fusing a TAL effector DNA-binding domain to a DNA cleavage domain (a nuclease which cuts DNA strands).