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Oil-immersion objective lenses look superficially identical to non-oil-immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...
Immersion oil has the same refraction as glass and improves the resolution of the observed specimen. Use of sample-staining methods for use in microbiology, such as simple stains (methylene blue, safranin, crystal violet) and differential stains (negative stains, flagellar stains, endospore stains).
However, for most applications it is recommended that oil immersion be used with fixed (dead) specimens because live cells require an aqueous environment, and the mixing of oil and water can cause severe spherical aberrations. For some applications silicone oil can be used to produce more accurate image reconstructions. Silicone oil is an ...
An oil immersion objective is an objective lens specially designed to be used in this way. The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion. Some microscopes also use other index-matching materials besides oil; see water ...
Used to detect specific DNA sequence in DNA samples: Molecular biology: Test cross: Used to determine whether an individual is homozygous or heterozygous dominant: Genetics: Voltage clamp: Used to measure the ion currents through the membranes of excitable cells, such as neurons, while holding the membrane voltage at a set level. [16 ...
Ramsey, however, suggests trying time blocking, a productivity method that involves scheduling specific chunks of time for individual tasks. (Example: Blocking out 10 minutes on your calendar so ...
The blurriness is reduced by a factor equal to the refractive index of the medium. For example, for water immersion using ultraviolet light at 193 nm wavelength, the index of refraction is 1.44. [3] The resolution enhancement from immersion lithography is about 30–40% depending on materials used.
Liquid-liquid extraction is a method used to separate an analyte from a complex mixture; with this method compounds separate based on their relative solubility in different immiscible liquid phases. [ 201 ] [ 202 ] To overcome some of the disadvantages associated with common bench top methods such as the shake-flask method, [ 203 ] Microfluidic ...