Search results
Results From The WOW.Com Content Network
Oil-immersion objective lenses look superficially identical to non-oil-immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...
An oil immersion objective is an objective lens specially designed to be used in this way. The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion. Some microscopes also use other index-matching materials besides oil; see water ...
In contrast to epifluorescence microscopy only a thin slice (usually a few hundred nanometers to a few micrometers) of the sample is illuminated perpendicularly to the direction of observation. For illumination, a laser light-sheet is used, i.e. a laser beam which is focused only in one direction (e.g. using a cylindrical lens). A second method ...
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
What links here; Related changes; Upload file; Special pages; Permanent link; Page information; Cite this page; Get shortened URL; Download QR code
However, this method is not always possible in live-cell imaging and may require additional intervention. Another method for reducing the effects of free radicals in the sample is the use of antifade reagents. Unfortunately, most commercial antifade reagents cannot be used in live-cell imaging because of their toxicity. [37]
In general, for immersed boundary methods and related variants, there is an active research community that is still developing new techniques and related software implementations and incorporating related techniques into simulation packages and CAD engineering software. For more details see below.
Liquid-liquid extraction is a method used to separate an analyte from a complex mixture; with this method compounds separate based on their relative solubility in different immiscible liquid phases. [ 201 ] [ 202 ] To overcome some of the disadvantages associated with common bench top methods such as the shake-flask method, [ 203 ] Microfluidic ...