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  2. Anti-dsDNA antibodies - Wikipedia

    en.wikipedia.org/wiki/Anti-dsDNA_antibodies

    Anti-dsDNA antibodies might also be created secondary to the production of antibodies to other proteins within the nucleosome. Mice that have T cells directed towards the nucleosome can elicit a response to other antigens such as dsDNA and histone via a mechanism known as antigen spreading. This effect can also occur after an infection causes ...

  3. DNA end resection - Wikipedia

    en.wikipedia.org/wiki/DNA_end_resection

    The presence of a ssDNA allows the broken end of the DNA to line up accurately with a matching sequence, so that it can be accurately repaired. [1] For HR pathway to occur in the S and G2 phases of the cell cycle, availability of a sister chromatid is required. [3] [4] 5′–3′ resection automatically links a DSB to the HR pathway. [2]

  4. DNA virus - Wikipedia

    en.wikipedia.org/wiki/DNA_virus

    Orthopoxvirus particles. A DNA virus is a virus that has a genome made of deoxyribonucleic acid (DNA) that is replicated by a DNA polymerase.They can be divided between those that have two strands of DNA in their genome, called double-stranded DNA (dsDNA) viruses, and those that have one strand of DNA in their genome, called single-stranded DNA (ssDNA) viruses. dsDNA viruses primarily belong ...

  5. Mung bean nuclease - Wikipedia

    en.wikipedia.org/wiki/Mung_bean_nuclease

    This enzyme work well at A↓pN, T ↓pN sites, and especially A↓pN sites are 100% degraded. However, it is difficult to degrade C↓pC, C↓pG site. Mung bean exonuclease is a nuclease derived from mung beans that removes nucleotides in a step-wise manner from single stranded DNA molecules and is used to remove such ssDNA from a mixture also ...

  6. Homologous recombination - Wikipedia

    en.wikipedia.org/wiki/Homologous_recombination

    The gene targeting method in knockout mice uses mouse embryonic stem cells to deliver artificial genetic material (mostly of therapeutic interest), which represses the target gene of the mouse by the principle of homologous recombination. The mouse thereby acts as a working model to understand the effects of a specific mammalian gene.

  7. Bacteriophage - Wikipedia

    en.wikipedia.org/wiki/Bacteriophage

    At this point they initiate the reproductive cycle, resulting in lysis of the host cell. As the lysogenic cycle allows the host cell to continue to survive and reproduce, the virus is replicated in all offspring of the cell. An example of a bacteriophage known to follow the lysogenic cycle and the lytic cycle is the phage lambda of E. coli. [55]

  8. Endonuclease - Wikipedia

    en.wikipedia.org/wiki/Endonuclease

    In mouse embryonic stem cells, an intermediate stage of crosslink repair involves production of double-strand breaks. [12] MUS81 / EME1 is a structure specific endonuclease involved in converting interstrand crosslinks to double-strand breaks in a DNA replication-dependent manner. [ 12 ]

  9. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA (dsDNA) is 0.020 (μg/mL) −1 cm −1, for single-stranded DNA (ssDNA) it is 0.027 (μg/mL) −1 cm −1, for single-stranded RNA (ssRNA) it is 0.025 (μg/mL) −1 cm −1 and for short single-stranded oligonucleotides it is dependent on the length and base ...