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The trophoblast (from Greek trephein: to feed; and blastos: germinator) is the outer layer of cells of the blastocyst. Trophoblasts are present four days after fertilization in humans. [ 1 ] They provide nutrients to the embryo and develop into a large part of the placenta .
Thus, there is a high demand for accurate cell lines to model primary placental trophoblasts. The immortalized cell line HTR-8/SVneo is commonly used to model EVTs. Newer multipotent trophoblast stem cell systems can be induced to differentiate into HLA-G+ EVT from CYT. [13]
The cytotrophoblast is considered to be the trophoblastic stem cell because the layer surrounding the blastocyst remains while daughter cells differentiate and proliferate to function in multiple roles. There are two lineages that cytotrophoblastic cells may differentiate through: fusion and invasive. The fusion lineage yields ...
Trophoblast cells also secrete factors to make the blastocoel. [25] After implantation, cytotrophoblast is the inner layer of the trophoblast, composed of stem cells which give rise to cells comprising the chorionic villi, placenta, and syncytiotrophoblast. After implantation, syncytiotrophoblast is the outermost layer of the trophoblast.
Image showing trophoblast differentiated into the two layers of cytotrophoblast and syncytiotrophoblast during implantation. It is the outer layer of the trophoblasts and actively invades the uterine wall, during implantation, rupturing maternal capillaries and thus establishing an interface between maternal blood and embryonic extracellular fluid, facilitating passive exchange of material ...
The fetal membranes are derived from the trophoblast layer (outer layer of cells) of the implanting blastocyst. [3] The trophoblast layer differentiates into amnion and the chorion, which then comprise the fetal membranes. [4] The amnion is the innermost layer and, therefore, contacts the amniotic fluid, the fetus and the umbilical cord. [5]
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