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The Phadebas Forensic Press test is used as a presumptive test for saliva. [5] Phadebas may be used to find saliva as a DNA source, or to identify the origin of a stain. The test is performed by placing paper bound with the Phadebas substrate to a sample, and applying pressure. [5]
An anti-α-amylase antibody is fixed at the test line. If human salivary amylase is present in the sample, it will form an antigen-antibody-colloidal gold complex upon deposition. These complexes react with the antibodies fixed at the test line to form a visible red line indicating the presence of human salivary α-amylase, and therefore, human ...
A common protocol used in the past for zymography of α-amylase activity was the so-called starch film protocol of W.W. Doane. Here a native PAGE gel was run to separate the proteins in a homogenate. Subsequently, a thin gel with starch dissolved (or more properly, suspended) in it was overlaid for a period of time on top of the original gel. [6]
A presumptive test to detect saliva is the alpha-amylase test also known as the Phadebas Test. [4] This detection technique is based on the activity of the enzyme alpha-amylase which breaks down starches from food into smaller oligosaccharide molecules, starting digestion in the mouth. [ 11 ]
The falling number method is uncomplicated, but requires an apparatus which follows the international standards. Such an apparatus consists of a water bath, a test tube, a stirring rod, and a stirring device. The test was performed manually when first employed, test instrumentation today is mostly automated. Flour samples can be analyzed directly.
The starch iodine test, a development of the iodine test, is based on colour change, as α-amylase degrades starch and is commonly used in many applications. A similar but industrially produced test is the Phadebas amylase test, which is used as a qualitative and quantitative test within many industries, such as detergents, various flour, grain ...
Phosphate tests test for phosphate; The sodium fusion test tests for the presence of nitrogen, sulfur, and halides in a sample; The Zerewitinoff determination tests for any acidic hydrogen; The Oddy test tests for acid, aldehydes, and sulfides; Gunzberg's test tests for the presence of hydrochloric acid; Kelling's test tests for the presence of ...
The procedure for Bradford protein assay is very easy and simple to follow. It is done in one step where the Bradford reagent is added to a test tube along with the sample. After mixing well, the mixture almost immediately changes to a blue color.