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MCF-7 cells. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old woman. [1] MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. [2] The Michigan Cancer Foundation is now known as the Barbara Ann Karmanos Cancer ...
Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination.
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
The E-SCREEN cell proliferation assay is performed with the human MCF-7 breast cancer cell line, an established estrogenic cell line that endogenously expresses ERα. [ 1 ] [ 3 ] [ 4 ] Human MCF-7 are cultivated in Dulbecco’s modified Eagle’s medium (DMEM) with fetal bovine serum (FBS) and phenol red as buffer tracer (culture medium), at 37 ...
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
In this analogy the field is a tissue culture dish, the plates the cells growing on it. While this was a reasonable and pragmatic approach automation of the whole process and the analysis makes possible the analysis of the whole population of living cells, so the whole football field can be measured.
Medium theory can be divided into microlevel and macrolevel issues. Microlevel medium theory explores the consequences of the choice of one medium over another in a particular situation, such as in initiating or ending a personal relationship, applying for a job, commanding troops, or interacting with one’s children.
Additionally, CD59 has been investigated as a target for immunotherapy when treating certain cancers such as breast cancer. Researchers have found that once CD59 had been targeted, there is an upregulation in fas and caspase-3, creating an increase in apoptosis and tumor growth suppression in MCF-7 cells. [13]