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MCF-7 cells. MCF-7 is a breast cancer cell line isolated in 1970 from a 69-year-old woman. [1] MCF-7 is the acronym of Michigan Cancer Foundation-7, referring to the institute in Detroit where the cell line was established in 1973 by Herbert Soule and co-workers. [2] The Michigan Cancer Foundation is now known as the Barbara Ann Karmanos Cancer ...
Microbial cell factory is an approach to bioengineering which considers microbial cells as a production facility in which the optimization process largely depends on metabolic engineering. [1] MCFs is a derivation of cell factories, which are engineered microbes and plant cells. [ 2 ]
Cell culture is a fundamental component of tissue culture and tissue engineering, as it establishes the basics of growing and maintaining cells in vitro. The major application of human cell culture is in stem cell industry, where mesenchymal stem cells can be cultured and cryopreserved for future use. Tissue engineering potentially offers ...
Culture media are typically formulated from basal media that provide cells with necessary carbohydrates, fats, proteins and salts. Once a cell consumes a sufficient amount, it divides and the population increases exponentially. Culture media can be supplemented with additives—for instance sera—that supply additional growth factors.
The first culture media was liquid media, designed by Louis Pasteur in 1860. [2] This was used in the laboratory until Robert Koch's development of solid media in 1881. [ 3 ] Koch's method of using a flat plate for his solid media was replaced by Julius Richard Petri's round box in 1887. [ 2 ]
An agar plate – an example of a bacterial growth medium*: Specifically, it is a streak plate; the orange lines and dots are formed by bacterial colonies.. A growth medium or culture medium is a solid, liquid, or semi-solid designed to support the growth of a population of microorganisms or cells via the process of cell proliferation [1] or small plants like the moss Physcomitrella patens. [2]
Standard cell culture media commonly consist of a basal medium supplemented with animal serum (such as fetal bovine serum, FBS) as a source of nutrients and other ill-defined factors. The technical disadvantages to using serum include its undefined nature, batch-to-batch variability in composition, and the risk of contamination.
The 3rd Annual Industrializing Cell-Based Meats & Seafood Summit is the only industry-led forum uniting key decision-makers from biotech and food tech, leading food and meat companies, and investors to discuss key operational and technical challenges for the development of cell-based meats and seafood.