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Phenol: The phenol used for biochemistry comes as a water-saturated solution with Tris buffer, as a Tris-buffered 50% phenol, 50% chloroform solution, or as a Tris-buffered 50% phenol, 48% chloroform, 2% isoamyl alcohol solution (sometimes called "25:24:1"). Phenol is naturally somewhat water-soluble, and gives a fuzzy interface, which is ...
The sample is dissolved in water, or a mixture of water and ethanol, and a few drops of neutral ferric chloride (FeCl 3) solution, which is prepared by adding de-ionised water. Add sodium hydroxide to the mixture until a permanent brown precipitate is formed.
Phenol (C 6 H 5 OH) is a water-soluble compound consisting of a phenyl group (-C 6 H 5) bonded to a hydroxyl group (-OH). Phenol extraction is a widely used technique for purifying nucleic acid samples from cell lysates. [1] To obtain nucleic acids, the cell must be lysed, and the nucleic acids separated from other cell components.
Phenol (also known as carbolic acid, phenolic acid, or benzenol) is an aromatic organic compound with the molecular formula C 6 H 5 OH. [5] It is a white crystalline solid that is volatile . The molecule consists of a phenyl group ( −C 6 H 5 ) bonded to a hydroxy group ( −OH ).
New peaks have appeared in the oxidised sample. Natural phenols are reactive species toward oxidation, notably the complex mixture of phenolics, found in food for example, can undergo autoxidation during the ageing process. Simple natural phenols can lead to the formation of B type proanthocyanidins in wines [17] or in model solutions.
The best-selling drug in the U.S., Acetaminophen, also known as Paracetamol, is a phenol. There are various classification schemes. [15]: 2 A commonly used scheme is based on the number of carbons and was devised by Jeffrey Harborne and Simmonds in 1964 and published in 1980: [15]: 2 [16]
Colorimetric analysis is a method of determining the concentration of a chemical element or chemical compound in a solution with the aid of a color reagent. It is applicable to both organic compounds and inorganic compounds and may be used with or without an enzymatic stage.
Presence of unsaturation and/or phenol or aniline in the sample is shown by disappearance of the deep brown coloration of bromine when it has reacted with the unknown sample. The formation of a brominated phenol (i.e. 2,4,6-tribromophenol) or aniline (i.e. 2,4,6-tribromoaniline) in form of a white precipitate indicates that the unknown was a ...