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Ehrlichia chaffeensis. E. chaffeensis causes human monocytic ehrlichiosis and is known to infect monocytes. [1] It has also been known to infect other cell types such as lymphocytes, atypical lymphocytes, myelocytes, and neutrophils, but monocytes appear to best harbor the infection.
Human monocytotropic ehrlichiosis [1] is a form of ehrlichiosis associated with Ehrlichia chaffeensis. [2] This bacterium is an obligate intracellular pathogen affecting monocytes and macrophages. [3]
Ehrlichia is a genus of Rickettsiales bacteria that are transmitted to vertebrates by ticks. These bacteria cause the disease ehrlichiosis , which is considered zoonotic , because the main reservoirs for the disease are animals.
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...
The diagnosis can be confirmed by using PCR. A peripheral blood smear can also be examined for intracytoplasmic inclusions called morulae. Doxycycline: No Anaplasma phagocytophilum: Human granulocytic anaplasmosis (HGA) PCR: Doxycycline: No Human metapneumovirus (hMPV) Human metapneumovirus infection No Ehrlichia chaffeensis: Human monocytic ...
Ehrlichia chaffeensis causes human monocytic ehrlichiosis. E. chaffeensis is most common in the south-central and southeastern states. Ehrlichia canis; Neorickettsia sennetsu; Ehrlichia muris eauclairensis [7] Ixodes scapularis is another type of tick that can spread Ehrlichiosis muris eauclairensis. [8] The latter three infections are not well ...
Ehrlichiosis ewingii infection [1] is an infectious disease caused by an intracellular bacteria, Ehrlichia ewingii. [2] The infection is transmitted to humans by the tick, Amblyomma americanum . This tick can also transmit Ehrlichia chaffeensis , the bacteria that causes human monocytic ehrlichiosis (HME).
The RFU measurements are used, for DNA profiling, in a real-time polymerase chain reaction (PCR). Two common methods for detection of products in real-time PCR are: (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA, and (2) sequence-specific DNA probes consisting of oligonucleotides that are labeled with a fluorescent reporter which permits detection only after ...