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In situ hybridization (ISH) is a type of hybridization that uses a labeled complementary DNA, RNA or modified nucleic acid strand (i.e., a probe) to localize a specific DNA or RNA sequence in a portion or section of tissue or if the tissue is small enough (e.g., plant seeds, Drosophila embryos), in the entire tissue (whole mount ISH), in cells ...
In plants, hybridization mostly generates speciation events, [5] and commonly produces polyploid species. Factors like polyploidy events also plays significant factors for understanding the hybridization events (Example: an F1 hybrid of Jatropha curcas x Ricinus communis ), [ 6 ] because these polyploids tend to have an advantage for the early ...
This is a list of plant hybrids created intentionally or by chance and exploited commercially in agriculture or horticulture. The hybridization event mechanism is documented where known, along with the authorities who described it.
Fluorescence in situ hybridization (FISH) refers to using fluorescently labeled probe to hybridize to cytogenetic cell preparations. In addition to standard preparations FISH can also be performed on: bone marrow smears; blood smears; paraffin embedded tissue preparations; enzymatically dissociated tissue samples; uncultured bone marrow ...
Hybridization without change in chromosome number is called homoploid hybrid speciation. [1] This is the situation found in most animal hybrids. For a hybrid to be viable, the chromosomes of the two organisms will have to be very similar, i.e., the parent species must be closely related, or else the difference in chromosome arrangement will ...
Hybridisation through grafting has the potential to create economically significant hybrid plants. [7] Graft hybridisation is a simple and practical method for breeding woody plants, particularly helpful for overcoming reproductive isolation and difficulties due to highly heterozygous genotypes. [9] [3] [6]
A similar hybridization technique is called a zoo blot. Bacterial FISH probes are often primers for the 16s rRNA region. FISH is widely used in the field of microbial ecology, to identify microorganisms. Biofilms, for example, are composed of complex (often) multi-species bacterial organizations. Preparing DNA probes for one species and ...
In situ hybridization is an alternate method in which a "probe," a synthetic nucleic acid with a sequence complementary to the mRNA of the gene, is added to the tissue. This probe is then chemically tagged so that it can be visualized later.