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A blood culture is a medical laboratory test used to detect bacteria or fungi in a person's blood. Under normal conditions, the blood does not contain microorganisms : their presence can indicate a bloodstream infection such as bacteremia or fungemia , which in severe cases may result in sepsis .
Two blood cultures drawn from separate sites of the body are often sufficient to diagnose bacteremia. [34] Two out of two cultures growing the same type of bacteria usually represents a real bacteremia, particularly if the organism that grows is not a common contaminant. [34] One out of two positive cultures will usually prompt a repeat set of ...
Blood culture bottle: Sodium polyanethol sulfonate (anticoagulant) and growth media for microorganisms: Usually drawn first for minimal risk of contamination. [1] Two bottles are typically collected in one blood draw; one for aerobic organisms and one for anaerobic organisms. [2] Blue ("light blue") Sodium citrate (weak calcium chelator ...
Blood agar plates (BAPs) contain mammalian blood (usually sheep or horse), typically at a 5–10% concentration. BAPs are enriched, and differential media is used to isolate fastidious organisms and detect hemolytic activity. β-Hemolytic activity will show lysis and complete digestion of red blood cell contents surrounding a colony.
The blood culture bottle contains transport media to preserve any microorganisms present while they are being transported to the laboratory for cultures. Because it is unknown whether the pathogens are anaerobic (living without oxygen) or aerobic (living with oxygen), blood is collected to test for both.
Hemolysis is the breakdown of red blood cells. The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. This is particularly useful in classifying streptococcal species. A substance that causes hemolysis is called a hemolysin.
A blood test is a laboratory analysis performed on a blood sample that is usually extracted from a vein in the arm using a hypodermic needle, or via fingerprick. Multiple tests for specific blood components, such as a glucose test or a cholesterol test , are often grouped together into one test panel called a blood panel or blood work .
Chocolate agar showing Francisella tularensis colonies Comparison of two culture media types used to grow Neisseria gonorrhoeae bacteria. Known as overgrowth, the nonselective chocolate agar medium on the left, due to its composition, allowed for the growth of organismal colonies other than those of N. gonorrhoeae, while the selective Thayer–Martin medium on the right, containing ...