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A fibrin clot formed in a test tube by the coagulase reaction. The tube test uses rabbit plasma that has been inoculated with a staphylococcal colony (i.e., Gram-positive cocci which are catalase positive). The tube is then incubated at 37 °C for 1.5 hours. If negative, then incubation is continued up to 18 hours.
[citation needed] S. lugdunensis may produce a bound coagulase (that is, the enzyme is bound to the cells), a property it shares with S. aureus, but unlike S. aureus, it does not produce a free coagulase. In the laboratory, it can give a positive slide-coagulase test but a negative tube-coagulase test.
Historically, biochemical tests have been an important tool used to discriminate between Staphylococcus species. [20] [8] Tests used to identify S. pseudintermedius specifically include DNase, [13] hyaluronidase, [22] coagulase, catalase, and acetoin production tests, amongst others. [8]
Staphylococcus schleiferi is a Gram-positive, cocci-shaped bacterium of the family Staphylococcaceae. [1] It is facultatively anaerobic, coagulase-variable, and can be readily cultured on blood agar where the bacterium tends to form opaque, non-pigmented colonies and beta (β) hemolysis. [2]
[12] [13] It is sensitive to novobiocin, providing an important test to distinguish it from Staphylococcus saprophyticus, which is coagulase-negative, as well, but novobiocin-resistant. [4] Similar to those of S. aureus, the cell walls of S. epidermidis have a transferrin-binding protein that helps the organism obtain iron from transferrin. The ...
Blood compatibility testing is routinely performed before a blood transfusion.The full compatibility testing process involves ABO and RhD (Rh factor) typing; screening for antibodies against other blood group systems; and crossmatching, which involves testing the recipient's blood plasma against the donor's red blood cells as a final check for incompatibility.