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The general formula for calculating the revolutions per minute (RPM) of a centrifuge is: R P M = g r {\displaystyle RPM={\sqrt {g \over r}}} , where g represents the relative centrifugal force (RCF) and r the radius from the center of the rotor to a point in the sample.
In centrifugation the clearing factor or k factor represents the relative pelleting efficiency of a given centrifuge rotor at maximum rotation speed. It can be used to estimate the time t {\displaystyle t} (in hours) required for sedimentation of a fraction with a known sedimentation coefficient s {\displaystyle s} (in svedbergs ):
A centrifuge is a device that uses centrifugal force to subject a specimen to a specified constant force - for example, to separate various components of a fluid. This is achieved by spinning the fluid at high speed within a container, thereby separating fluids of different densities (e.g. cream from milk) or liquids from solids.
Typical differential centrifugation parameters for a biological sample [2] (path length of centrifugation ≈1–5 cm) Sample input G force Time Instrument needed Pellet contents Supernatant contents Unlysed (eukaryotic) cells 100 x g 5 min Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Intact (eukaryotic) cells, macroscopic debris
For example, in an Earth-bound reference system (where the earth is represented as stationary), the fictitious force (the net of Coriolis and centrifugal forces) is enormous and is responsible for the Sun orbiting around the Earth. This is due to the large mass and velocity of the Sun (relative to the Earth).
Diagram of a gas centrifuge with countercurrent flow, used for separating isotopes of uranium. A gas centrifuge is a device that performs isotope separation of gases. A centrifuge relies on the principles of centrifugal force accelerating molecules so that particles of different masses are physically separated in a gradient along the radius of a rotating container.
With a 3 phase decanter centrifuge, it is possible to separate 3 phases from each other in one process step only. For example, two liquids which cannot be mixed because of different densities (e.g. oil and water) are separated from a solids phase. The heavy liquid (water) collects in the middle between the oil and the solids layer.
An ultracentrifuge is a centrifuge optimized for spinning a rotor at very high speeds, capable of generating acceleration as high as 1 000 000 g (approx. 9 800 km/s²). [1] There are two kinds of ultracentrifuges, the preparative and the analytical ultracentrifuge.
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