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The vector itself generally carries a DNA sequence that consists of an insert (in this case the transgene) and a larger sequence that serves as the "backbone" of the vector. The purpose of a vector which transfers genetic information to another cell is typically to isolate, multiply, or express the insert in the target cell.
Vector (C++), a type in the C++ Standard Template Library; Euclidean vector, a geometric object with a direction and magnitude Vector graphics, images defined by geometric primitives as opposed to bitmaps; Vector monitor, a display device used for early computers; Vector game, any video game that uses a vector graphics display
In epidemiology, a disease vector is any living [1] agent that carries and transmits an infectious pathogen such as a parasite or microbe, to another living organism. [ 2 ] [ 3 ] Agents regarded as vectors are mostly blood-sucking insects such as mosquitoes.
The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. [1] The goal of a well-designed expression vector is the efficient production of protein, and this may be achieved by the production of significant amount of stable ...
Viral vector manufacturing methods often vary by vector, although most utilize an adherent or suspension-based system with mammalian cells. [72] For viral vector production on a smaller, laboratory setting, static cell culture systems like Petri dishes are typically used.
A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. [1] The cloning vector may be DNA taken from a virus, the cell of a higher organism, or it may be the plasmid of a bacterium.
An expression cassette is a distinct component of vector DNA consisting of a gene and regulatory sequence to be expressed by a transfected cell. [1] In each successful transformation, the expression cassette directs the cell's machinery to make RNA and protein(s). Some expression cassettes are designed for modular cloning of protein-encoding ...
The choice of vector for molecular cloning depends on the choice of host organism, the size of the DNA to be cloned, and whether and how the foreign DNA is to be expressed. [7] The DNA segments can be combined by using a variety of methods, such as restriction enzyme/ligase cloning or Gibson assembly .