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The first frog egg extract was reported in 1983 by Lohka and Masui. [1] This pioneering work used eggs of the Northern leopard frog Rana pipiens to prepare an extract. Later, the same procedure was applied to eggs of Xenopus laevis, becoming popular for studying cell cycle progression and cell cycle-dependent cellular events. [2]
The CO type of recombination involves the intermediate formation of two "Holliday junctions" indicated in the lower right of the figure by two X-shaped structures in each of which there is an exchange of single strands between the two participating chromatids. This pathway is labeled in the figure as the DHJ (double-Holliday junction) pathway.
There are two popular and overlapping theories that explain the origins of crossing-over, coming from the different theories on the origin of meiosis.The first theory rests upon the idea that meiosis evolved as another method of DNA repair, and thus crossing-over is a novel way to replace possibly damaged sections of DNA. [9]
Meiosis (/ m aɪ ˈ oʊ s ɪ s / ⓘ; from Ancient Greek μείωσις (meíōsis) 'lessening', (since it is a reductional division) [1] [2] is a special type of cell division of germ cells in sexually-reproducing organisms that produces the gametes, the sperm or egg cells. It involves two rounds of division that ultimately result in four ...
These chromosomes, carrying genetic information, align in the equator of the cell between the spindle poles at the metaphase plate, before being separated into each of the two daughter nuclei. This alignment marks the beginning of metaphase. [2] Metaphase accounts for approximately 4% of the cell cycle's duration. [citation needed]
Ndt80 stimulates the expression of the B-type cyclin Clb-1, which greatly interacts with Cdk1 during meiotic divisions. [24] Active complexes of Clb-1 with Cdk1 play a large role in triggering the events of the first meiotic division, and their activity is restricted to meiosis 1. [25]
Mitosis (/ m aɪ ˈ t oʊ s ɪ s /) is a part of the cell cycle in which replicated chromosomes are separated into two new nuclei. Cell division by mitosis is an equational division which gives rise to genetically identical cells in which the total number of chromosomes is maintained. [1]
Cell synchronization is a process by which cells in a culture at different stages of the cell cycle are brought to the same phase. Cell synchrony is a vital process in the study of cells progressing through the cell cycle as it allows population-wide data to be collected rather than relying solely on single-cell experiments.