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Fluorescein is used to help in the diagnosis of a number of eye problems. [3] When applied as a drop or within a strip of paper to the surface of the eye it is used to help detect eye injuries such as foreign bodies and corneal abrasions .
Fluorescein 2% [2] is instilled into the lower fornix of patient's eye. Alternatively, impregnated fluorescein strip moistened with preservative (benzalkonium chloride) free saline solution can also be used. [6] The eye is examined under a slit lamp with a low magnification and a broad beam covering the whole cornea. The lamp is switched to a ...
The fluorescein is administered intravenously in intravenous fluorescein angiography (IVFA) and orally in oral fluorescein angiography (OFA). The test is a dye tracing method. The fluorescein dye also reappears in the patient urine, causing the urine to appear darker, and sometimes orange. [2] It can also cause discolouration of the saliva.
The fluorescein is examined with a cobalt blue filter. At this point, the fluorescein appears green in color. Any changes in color or surface of the fluorescence area indicate the presence of corneal leakage. The test is contraindicated in obvious globe rupture, Full-thickness eye laceration, and fluorescein hypersensitivity. [1]
Fluorescein can be introduced to a plant's veins through the roots or a cut stem. The dye is able to be taken up into the plant the same way as water and moves from the roots to the top of the plant due to a transpirational pull. [29] The fluorescein that has been taken up into the plant can be visualized under a fluorescent microscope.
Diagnosis is often by slit lamp examination after fluorescein dye has been applied. [1] More significant injuries like a corneal ulcer, globe rupture, recurrent erosion syndrome, and a foreign body within the eye should be ruled out. [1] Prevention includes the use of eye protection. [1] Treatment is typically with antibiotic ointment. [1]
Another test involves fluorescein eye drops, which contain a dye that is placed in the eye. The dye should drain with the tears through the lacrimal duct into the nose within 2 minutes. If patients do not have enough tears to flush the dye into the nose, this time will be longer. A new test is also available to more accurately measure the flow ...
In pure aqueous tear deficiency dry eye patients, fluorescein stains in the interpalpebral exposure zone. In mechanical dry eye patients, fluorescein staining can be seen by pulling down the lower lid spread to the non-exposure zone. A tear-clearance test can also detect irregularities in the tear film. [6]