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The hemocytometer (or haemocytometer, or Burker's chamber) is a counting-chamber device originally designed and usually used for counting blood cells. [ 1 ] The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a precision volume chamber.
A counting chamber. A counting chamber, is a microscope slide that is especially designed to enable cell counting. Hemocytometers and Sedgewick Rafter counting chambers are two types of counting chambers. The hemocytometer has two gridded chambers in its middle, which are covered with a special glass slide when counting.
Otto Neubauer (8 April 1874 – 24 November 1957) was a Bohemia-born physician and biochemist who was responsible for several clinical diagnostic innovations including the Neubauer-Fischer test to evaluate kidney function and the Neubauer counting chamber.
Through the work of Karl von Vierordt, Louis-Charles Malassez, Karl Bürker and others blood cell concentration could by the late 19th century be accurately measured using a blood cell counting chamber, the hemocytometer, and an optical microscope. [3] [4] Until the 1950s the hemocytometer was the standard method to count blood cells. [5]
Determining the viable cell count is important for calculating dilutions required for the passaging of cells, as well as determining the size and number of flasks needed during growth time. It is also vital when seeding plates for assays, such as the plaque assay , [ 2 ] because the plates need a known number of live replicating cells for the ...
"Often known as the 'Pope of Biology', the hemocytometer is often prayed to in order to receive good results. As it is such an important instrument for many biological experiments, breaking or misuse of a haemocytometer will result in bad luck in science for 8 years, the total number of quadrants on a haemocytometer."
A Registered Dietitian's Guide to Counting Macros. Isadora Baum, Amy Fischer M.S., R.D., C.D.N. December 9, 2022 at 12:33 PM. How to Count Macros, According to Dietitians Thana Prasongsin - Getty ...
MCHC can be normal even when hemoglobin production is decreased (such as in iron deficiency) due to a calculation artifact. MCHC can be elevated ("hyperchromic") in hereditary spherocytosis, sickle cell disease and homozygous hemoglobin C disease, depending upon the hemocytometer. [4] [5] MCHC can be elevated in some megaloblastic anemias. MCHC ...