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  2. Nucleic acid quantitation - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_quantitation

    The ratio of the absorbance at 260 and 280 nm (A 260/280) is used to assess the purity of nucleic acids. For pure DNA, A 260/280 is widely considered ~1.8 but has been argued to translate - due to numeric errors in the original Warburg paper - into a mix of 60% protein and 40% DNA. [6] The ratio for pure RNA A 260/280 is ~2.0. These ratios are ...

  3. Qubit fluorometer - Wikipedia

    en.wikipedia.org/wiki/Qubit_fluorometer

    Upon binding to DNA, the dye molecules assume a more rigid shape and increase in fluorescence by several orders of magnitude, most likely due to intercalation between the bases. [ 9 ] [ 10 ] The Qubit fluorometer, a device designed to measure fluorescence signals from samples, operates by correlating these signals with known concentrations of ...

  4. Genetic testing - Wikipedia

    en.wikipedia.org/wiki/Genetic_testing

    Genetic testing, also known as DNA testing, is used to identify changes in DNA sequence or chromosome structure. Genetic testing can also include measuring the results of genetic changes, such as RNA analysis as an output of gene expression , or through biochemical analysis to measure specific protein output. [ 1 ]

  5. RiboGreen - Wikipedia

    en.wikipedia.org/wiki/RiboGreen

    RiboGreen is a proprietary fluorescent dye that is used in the detection and quantification of nucleic acids, including both RNA and DNA. It is synthesized and marketed by Molecular Probes/Invitrogen (a division of Life Technologies, now part of Thermo Fisher Scientific) of Eugene, Oregon, United States. In its free form, RiboGreen exhibits ...

  6. After DNA test, 2 women discover they were switched at birth ...

    www.aol.com/lifestyle/dna-test-2-women-discover...

    After DNA test, 2 women discover they were switched at birth — nearly 60 years ago. Rachel Paula Abrahamson. March 1, 2022 at 9:16 AM. When Tina Ennis took an Ancestry.com DNA test in 2019, she ...

  7. Nucleic acid methods - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_methods

    DNA sequencing; Expression cloning; Fluorescence in situ hybridization; Lab-on-a-chip; Comparison of nucleic acid simulation software; Northern blot; Nuclear run-on assay; Radioactivity in the life sciences; Southern blot; Differential centrifugation (sucrose gradient) Toeprinting assay; Several bioinformatics methods, as seen in list of RNA ...

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