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The Gerber method is a primary and historic chemical test to determine the fat content of substances, most commonly milk and cream. [1] The Gerber method is the primary testing method in Europe and much of the world. [2] The fairly similar Babcock test is used primarily in the United States, although the Gerber method also enjoys significant ...
Emulsion test. The emulsion test is a simple method used educational settings to determine the presence of lipids using wet chemistry. The procedure is for the sample to be suspended in ethanol, allowing lipids present to dissolve (lipids are soluble in alcohols). The liquid (alcohol with dissolved fat) is then decanted into water. Since lipids ...
Specifically, the test consisted of the following steps: [ 6 ][ 7 ][ 8 ] Place 17.6 mL (18 grams) of milk into a Babcock bottle. Add 17.6 mL of 90-92% sulfuric acid at 15-20°C. Spin the flask in a hand-cranked centrifuge for 5 minutes. Add water at 60°C until the fat layer is all in the neck.
Iodine value. In chemistry, the iodine value (IV; also iodine absorption value, iodine number or iodine index) is the mass of iodine in grams that is consumed by 100 grams of a chemical substance. Iodine numbers are often used to determine the degree of unsaturation in fats, oils and waxes. In fatty acids, unsaturation occurs mainly as double ...
Alternatively, authors have reported using the dye saturated in isopropyl alcohol, 95% ethanol, or 0.05% by weight in acetone:ethanol:water (50:35:15). [citation needed] The idea is to use a moderately apolar solvent to solubilize the dye allowing it to partition into the highly apolar fat without the solvent solubilizing the fat to be stained.
A Babcock bottle is a clear glass flask with a long graduated neck, used in the Babcock test to evaluate the cream contents of milk. [1] It is also called a Babcock milk test bottle, milk test bottle, cream test bottle, and other similar names. [2][3][4][5] This bottle (or variations thereof) may also be used to estimate the amount of a lighter ...
A pancreatic elastase test is a diagnostic tool for endocrine pancreatic insufficiency. Abnormal results don’t always indicate EPI. ... fats, and carbohydrates ... Excessive alcohol use. Human ...
Molisch test (using α-napthol) indicating a positive result (see purple ring). Molisch's test is a sensitive chemical test, named after Austrian botanist Hans Molisch, for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol (usually α-naphthol, though other ...