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Fluorescence imaging with one nanometer accuracy (FIONA): utilizes total internal reflection illumination to reduce noise and increase brightness of fluorophores [5] Calcium imaging: technique that utilizes fluorescent molecules called calcium indicators that change in fluorescence when bound to Ca 2+ ions. This is a key part in seeing when ...
ICG absorbs mainly between 600 nm and 900 nm and emits fluorescence between 750 nm and 950 nm. The large overlapping of the absorption and fluorescence spectra leads to a marked reabsorption of the fluorescence by ICG itself. The fluorescence spectrum is very wide. Its maximum values are approx. 810 nm in water and approx. 830 nm in blood.
Not fluorescent by itself, it can bind selectively a fluorogenic chromophore derived from 4-hydroxybenzylidene rhodanine, which is itself non fluorescent unless bound. Once bound, the pair of molecules goes through a unique fluorogen activation mechanism based on two spectroscopic changes, increase of fluorescence quantum yield and absorption ...
Imaging lenses and digital cameras (CCD or CMOS) are used to produce the final image. Live video processing can also be performed to enhance contrast during fluorescence detection and improve signal-to-background ratio. In recent years a number of commercial companies have emerged to offer devices specializing in fluorescence in the NIR ...
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
The quest for fluorescent probes with a high specificity that also allow live imaging of plant cells is ongoing. [7] There are many fluorescent molecules called fluorophores or fluorochromes such as fluorescein, Alexa Fluors, or DyLight 488, which can be chemically linked to a different molecule which binds the target of interest within the sample.
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