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Flow cytometry (FC) is a technique used to detect and measure the physical and chemical characteristics of a population of cells or particles. [1] [2] [3] [4]In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument.
Monoclonal CLL/SLL phenotype B cells have been found using sensitive flow cytometry methods in various tissues. [3] They have been identified as infiltrates in 1.9% of liver biopsies and 0.4% of prostate tissues obtained at prostatectomy .
The process of immunological B-cell maturation involves transformation from an undifferentiated B cell to one that secretes antibodies with particular specificity. [1] This differentiation and activation of the B cell occurs most rapidly after exposure to antigen by antigen-presenting cells in the reticuloendothelial system, and under modulation by T cells, and is closely intertwined with ...
In the flow cytometry-based screening, a mixture of antigen-negative cells and antigen-positive cells is used as the antigen to be tested for each hybridoma supernatant sample. [ 4 ] The B cell that produces the desired antibodies can be cloned to produce many identical daughter clones.
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, and the Interleukin-6 receptor. In humans, CD27 is a good marker for plasma cells; naïve B cells are CD27−, memory B-cells are CD27+ and plasma cells are CD27++. [5] The surface antigen CD138 (syndecan-1) is expressed at high levels. [6]
Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.Before analysis, the cells are usually permeabilised and treated with a fluorescent dye that stains DNA quantitatively, such as propidium iodide (PI) or 4,6-diamidino-2-phenylindole (DAPI).
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