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KOH test on a vaginal wet mount, showing slings of pseudohyphae of Candida albicans surrounded by round vaginal epithelial cells, conferring a diagnosis of candidal vulvovaginitis. The KOH test , also known as a potassium hydroxide preparation or KOH prep , is a quick, inexpensive fungal test to differentiate dermatophytes and Candida albicans ...
Candida albicans is one of the most commonly isolated species and can cause infections (candidiasis or thrush) in humans and other animals. In winemaking, some species of Candida can potentially spoil wines. [4] Many species are found in gut flora, including C. albicans in mammalian hosts, whereas others live as endosymbionts in insects.
Sabouraud agar or Sabouraud dextrose agar (SDA) is a type of agar growth medium containing peptones. [1] It is used to cultivate dermatophytes and other types of fungi , and can also grow filamentous bacteria such as Nocardia .
While Candida albicans is the most common yeast species associated with vaginal thrush, infection by other types of yeast can produce similar symptoms. A Hungarian study of 370 patients with confirmed vaginal yeast infections identified the following types of infection: [18] Candida albicans: 85.7%; Non-albicans Candida (8 species): 13.2%
The type of medium in the agar plates is also important. Chromagar Candida is a differential medium used to identify different Candida species. When grown on this medium, C. albicans is light green, C. tropicalis is steel blue with purple around the edges, and C. krusei is rose pink with white around the edges. [ 7 ]
Gram stain of Candida albicans from a vaginal swab, showing hyphae, and chlamydospores, which are 2–4 μm in diameter.. A chlamydospore is the thick-walled large resting spore of several kinds of fungi, including Ascomycota such as Candida, [1] Basidiomycota such as Panus, [2] and various Mortierellales species. [3]
Saccharomycotina is a subdivision (subphylum) of the division (phylum) Ascomycota in the kingdom Fungi. [2] [3] It comprises most of the ascomycete yeasts.The members of Saccharomycotina reproduce by budding and they do not produce ascocarps (fruiting bodies).
A third technique is using sterile glass beads to plate out cells. In this technique, cells are grown in a liquid culture, in which a small volume is pipetted on the agar plate and then spread out with the beads. Replica plating is another technique used to plate out cells on agar plates. These four techniques are the most common, but others ...