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Celloscope automated cell counter was developed in the 1950s for enumeration of erythrocytes, leukocytes, and thrombocytes in blood samples. [1] Together with the Coulter counter, the Celloscope analyzer can be considered one of the predecessors of today's automated hematology analyzers, as the principle of the electrical impedance method is still utilized in cell counters installed in ...
The Coulter counter is the commercial term for the technique known as resistive pulse sensing or electrical zone sensing. The apparatus is based on the Coulter principle named after its inventor, Wallace H. Coulter. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.
Hematology analyzers are used to conduct a complete blood count (CBC), which is usually the first test requested by physicians to determine a patient's general health status. [5] A complete blood count includes red blood cell (RBC), white blood cell (WBC), hemoglobin, and platelet counts, as well as hematocrit levels.
The passage of cells through the current changes the impedance between the terminals (the Coulter principle). [6] A lytic reagent is added to the blood solution to selectively lyse the red cells (RBCs), leaving only white cells (WBCs), and platelets intact. Then the solution is passed through a second detector.
In electrical engineering, impedance is the opposition to alternating current presented by the combined effect of resistance and reactance in a circuit. [1]Quantitatively, the impedance of a two-terminal circuit element is the ratio of the complex representation of the sinusoidal voltage between its terminals, to the complex representation of the current flowing through it. [2]
An impedance analyzer is a type of electronic test equipment used to measure complex electrical impedance as a function of test frequency. Impedance is an important parameter used to characterize electronic components, electronic circuits, and the materials used to make components. Impedance analysis can also be used to characterize materials ...
Four-point measurement of resistance between voltage sense connections 2 and 3. Current is supplied via force connections 1 and 4. In electrical engineering, four-terminal sensing (4T sensing), 4-wire sensing, or 4-point probes method is an electrical impedance measuring technique that uses separate pairs of current-carrying and voltage-sensing electrodes to make more accurate measurements ...
The AC impedance of the cell-covered electrode is then measured at one or several frequencies as a function of time. Due to the insulating properties of their membranes the cells behave like dielectric particles so that the impedance increases with increasing coverage of the electrode until a confluent (i.e. continuous) layer of cells is ...