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Celloscope automated cell counter was developed in the 1950s for enumeration of erythrocytes, leukocytes, and thrombocytes in blood samples. [1] Together with the Coulter counter, the Celloscope analyzer can be considered one of the predecessors of today's automated hematology analyzers, as the principle of the electrical impedance method is still utilized in cell counters installed in ...
The Coulter counter is the commercial term for the technique known as resistive pulse sensing or electrical zone sensing. The apparatus is based on the Coulter principle named after its inventor, Wallace H. Coulter. A typical Coulter counter has one or more microchannels that separate two chambers containing electrolyte solutions.
The Sysmex XE-2100 is a haematology automated analyser, used to quickly perform full blood counts and reticulocyte counts. It is made by the Sysmex Corporation. It can be run on its own, or connected to a blood film making and staining unit. Racks of blood go in on a tray on the right, and come out the left side.
The Coulter principle uses electrical impedance measurements to count blood cells and determine their sizes; it is a technology that remains in use in many automated analyzers. Further research in the 1970s involved the use of optical measurements to count and identify cells, which enabled the automation of the white blood cell differential.
Hematology analyzers are used to conduct a complete blood count (CBC), which is usually the first test requested by physicians to determine a patient's general health status. [5] A complete blood count includes red blood cell (RBC), white blood cell (WBC), hemoglobin, and platelet counts, as well as hematocrit levels.
The passage of cells through the current changes the impedance between the terminals (the Coulter principle). [6] A lytic reagent is added to the blood solution to selectively lyse the red cells (RBCs), leaving only white cells (WBCs), and platelets intact. Then the solution is passed through a second detector.
Wallace H. Coulter (February 17, 1913 – August 7, 1998) was an American electrical engineer, inventor, and businessman.The best known of his 85 patents is the Coulter principle, which provides a method for counting and sizing microscopic particles suspended in fluid.
Often, data obtained by electrochemical impedance spectroscopy (EIS) is expressed graphically in a Bode plot or a Nyquist plot. Impedance is the opposition to the flow of alternating current (AC) in a complex system. A passive complex electrical system comprises both energy dissipater and energy storage elements.