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A stem cell line is a group of stem cells that is cultured in vitro and can be propagated indefinitely. Stem cell lines are derived from either animal or human tissues and come from one of three sources: embryonic stem cells, adult stem cells, or induced pluripotent stem cells. They are commonly used in research and regenerative medicine.
An immortalised cell line is a population of cells from a multicellular organism that would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division.
A cell strain is derived either from a primary culture or a cell line by the selection or cloning of cells having specific properties or characteristics which must be defined. Cell strains are cells that have been adapted to culture but, unlike cell lines, have a finite division potential.
The degree of HeLa cell contamination among other cell types is unknown, because few researchers test the identity or purity of already established cell lines. It has been shown that a substantial fraction of in vitro cell lines are contaminated with HeLa cells; estimates range from 10% to 20%.
Suspension cell culture is commonly used to culture nonadhesive cell lines like hematopoietic cells, plant cells, and insect cells. [1] While some cell lines are cultured in suspension, the majority of commercially available mammalian cell lines are adherent.
MRC-5 cell. MRC-5 (Medical Research Council cell strain 5) is a diploid cell culture line composed of fibroblasts, originally developed from the lung tissue of a 14-week-old aborted Caucasian male fetus.
SH-SY5Y is a human derived cell line used in scientific research. The original cell line, called SK-N-SH, from which it was subcloned was isolated from a bone marrow biopsy taken from a four-year-old female with neuroblastoma. SH-SY5Y cells are often used as in vitro models of neuronal function and
A hybrid cell line is a fusion of cells from two different cell types. When the membrane of two cells merge, the nuclei combine to form a polykaryote (poly- multiple; karyon- chromosome ). [ 1 ] These fusions can happen spontaneously as in the case of tumor hybrid cells, or may be induced by a variety of laboratory techniques.