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The specimen is illuminated with light of a specific wavelength (or wavelengths) which is absorbed by the fluorophores, causing them to emit light of longer wavelengths (i.e., of a different color than the absorbed light). The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter.
Ultraviolet light is a form of electromagnetic radiation that ranges in wavelengths from 10 nm to 400 nm. [6] This wavelength is shorter than visible light but longer than X-rays. [6] As it sits on the lower edge of visible light, is what gives its name. The most effective wavelength of UV light is approximately 250 nm. [6]
The wavelength of light is then selected by the slit on the upper right corner. An optical spectrometer (spectrophotometer, spectrograph or spectroscope) is an instrument used to measure properties of light over a specific portion of the electromagnetic spectrum, typically used in spectroscopic analysis to identify materials. [1]
Fluorescence interference contrast (FLIC) microscopy is a microscopic technique developed to achieve z-resolution on the nanometer scale.. FLIC occurs whenever fluorescent objects are in the vicinity of a reflecting surface (e.g. Si wafer).
Full-spectrum light is light that covers the electromagnetic spectrum from infrared to near-ultraviolet, or all wavelengths that are useful to plant or animal life; in particular, sunlight is considered full spectrum, even though the solar spectral distribution reaching Earth changes with time of day, latitude, and atmospheric conditions.
Far-red light is a range of light at the extreme red end of the visible spectrum, just before infrared light. Usually regarded as the region between 700 and 750 nm wavelength , it is dimly visible to human eyes.