Ads
related to: whole genome bisulfite sequencing
Search results
Results From The WOW.Com Content Network
The whole genome sequencing technique was first applied to the DNA methylation mapping at single nucleotide resolution to Arabidopsis thaliana in 2008, and shortly after in 2009, the first single-base-resolution DNA methylation map of the entire human genome was created using whole genome bisulfite sequencing.
Bisulfite [1] sequencing (also known as bisulphite sequencing) is the use of bisulfite treatment of DNA before routine sequencing to determine the pattern of methylation. DNA methylation was the first discovered epigenetic mark, and remains the most studied.
Single cell DNA genome sequencing quantifies DNA methylation. This is similar to single cell genome sequencing, but with the addition of a bisulfite treatment before sequencing. Forms include whole genome bisulfite sequencing, [ 4 ] [ 5 ] and reduced representation bisulfite sequencing [ 6 ] [ 7 ]
Whole genome bisulfite sequencing, also known as BS-Seq, which is a high-throughput genome-wide analysis of DNA methylation. It is based on the aforementioned sodium bisulfite conversion of genomic DNA, which is then sequenced on a Next-generation sequencing platform. The sequences obtained are then re-aligned to the reference genome to ...
Reduced representation bisulfite sequencing (RRBS) is an efficient and high-throughput technique for analyzing the genome-wide methylation profiles on a single nucleotide level. It combines restriction enzymes and bisulfite sequencing to enrich for areas of the genome with a high CpG content.
Whole-Genome Bisulfite Sequencing (WGBS) to examine DNA methylation in cellular subpopulations isolated from human brain tissue. This analysis is important, because DNA methylation differences between neuronal and non-neuronal populations have been widely reported and many neuropsychiatric diseases preferentially affect neuronal subpopulations ...
Ad
related to: whole genome bisulfite sequencing9.0/10 (380 reviews)