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This enzyme work well at A↓pN, T ↓pN sites, and especially A↓pN sites are 100% degraded. However, it is difficult to degrade C↓pC, C↓pG site. Mung bean exonuclease is a nuclease derived from mung beans that removes nucleotides in a step-wise manner from single stranded DNA molecules and is used to remove such ssDNA from a mixture also ...
Restriction endonucleases may be found that cleave standard dsDNA (double-stranded DNA), or ssDNA (single-stranded DNA), or even RNA. [citation needed] This discussion is restricted to dsDNA; however, the discussion can be extended to the following: Standard dsDNA; Non-standard DNA; Holliday junctions
Differences in relative abundance of transcripts are highlighted, as are genetic differences between species. The technique relies on the removal of dsDNA formed by hybridization between a control and test sample, thus eliminating cDNAs or gDNAs of similar abundance, and retaining differentially expressed, or variable in sequence, transcripts ...
Nucleic acid thermodynamics is the study of how temperature affects the nucleic acid structure of double-stranded DNA (dsDNA). The melting temperature (T m) is defined as the temperature at which half of the DNA strands are in the random coil or single-stranded (ssDNA) state. T m depends on the length of the DNA molecule and its specific ...
At a wavelength of 260 nm, the average extinction coefficient for double-stranded DNA (dsDNA) is 0.020 (μg/mL) −1 cm −1, for single-stranded DNA (ssDNA) it is 0.027 (μg/mL) −1 cm −1, for single-stranded RNA (ssRNA) it is 0.025 (μg/mL) −1 cm −1 and for short single-stranded oligonucleotides it is dependent on the length and base ...
Interactions between microRNAs and complementary sequences on genes and even pseudogenes that share sequence homology are thought to be a back channel of communication regulating expression levels between paralogous genes (genes having a similar structure indicating divergence from a common ancestral gene).
RNA silencing describes several mechanistically related pathways which are involved in controlling and regulating gene expression. [5] [6] [7] RNA silencing pathways are associated with the regulatory activity of small non-coding RNAs (approximately 20–30 nucleotides in length) that function as factors involved in inactivating homologous sequences, promoting endonuclease activity ...
The gene targeting method in knockout mice uses mouse embryonic stem cells to deliver artificial genetic material (mostly of therapeutic interest), which represses the target gene of the mouse by the principle of homologous recombination. The mouse thereby acts as a working model to understand the effects of a specific mammalian gene.