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This mass shift can be detected by a mass spectrometer as indicated by the depicted mass spectra. When both samples are combined, the ratio of peak intensities in the mass spectrum reflects the relative protein abundance. In this example, the labeled protein has the same abundance in both samples (ratio 1).
Schematic diagram of the combined ECD FTICRMS and IRMPD experimental setup. Electron-capture dissociation (ECD) is a method of fragmenting gas-phase ions for structure elucidation of peptides and proteins in tandem mass spectrometry. It is one of the most widely used techniques for activation and dissociation of mass selected precursor ion in ...
In physics, a mass balance, also called a material balance, is an application of conservation of mass [1] to the analysis of physical systems.By accounting for material entering and leaving a system, mass flows can be identified which might have been unknown, or difficult to measure without this technique.
A quadrupole time-of-flight hybrid tandem mass spectrometer. Tandem mass spectrometry, also known as MS/MS or MS 2, is a technique in instrumental analysis where two or more stages of analysis using one or more mass analyzer are performed with an additional reaction step in between these analyses to increase their abilities to analyse chemical samples. [1]
A typical workflow of a peptide mass fingerprinting experiment. Peptide mass fingerprinting (PMF), also known as protein fingerprinting, is an analytical technique for protein identification in which the unknown protein of interest is first cleaved into smaller peptides, whose absolute masses can be accurately measured with a mass spectrometer such as MALDI-TOF or ESI-TOF. [1]
A mass spectrum is a histogram plot of intensity vs. mass-to-charge ratio (m/z) in a chemical sample, [1] usually acquired using an instrument called a mass spectrometer. Not all mass spectra of a given substance are the same; for example, some mass spectrometers break the analyte molecules into fragments ; others observe the intact molecular ...
In the life sciences, mass flow, also known as mass transfer and bulk flow, is the movement of fluids down a pressure or temperature gradient. [1] As such, mass flow is a subject of study in both fluid dynamics and biology. Examples of mass flow include blood circulation and transport of water in vascular plant tissues. Mass flow is not to be ...
The conservation of mass was obscure for millennia because of the buoyancy effect of the Earth's atmosphere on the weight of gases. For example, a piece of wood weighs less after burning; [17] this seemed to suggest that some of its mass disappears, or is transformed or lost. Careful experiments were performed in which chemical reactions such ...