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Oil immersion objective lenses look superficially identical to non-oil immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...
In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0. Objective lenses with a higher NA can be used but ...
The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion . Some microscopes also use other index-matching materials besides oil; see water immersion objective and solid immersion lens .
Oil immersion is a technique that can increase image resolution by immersing the lens and the specimen in oil with a high refractive index. Since light bends when it passes between media with different refractive indexes, by placing oil with the same refractive index as glass between the lens and the slide, two transitions between refractive ...
Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
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This index is determined by comparing two minerals directly, or comparing a mineral to a reference material such as Canada Balsam or an oil of known refractive index (oil immersion). When permanently mounted to a slide under a cover slip, the mounting medium is normally chosen to have the same refractive index as Canada Balsam ( n =1.55) to ...