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Optical mapping [1] is a technique for constructing ordered, genome-wide, high-resolution restriction maps from single, stained molecules of DNA, called "optical maps". By mapping the location of restriction enzyme sites along the unknown DNA of an organism, the spectrum of resulting DNA fragments collectively serves as a unique "fingerprint" or "barcode" for that sequence.
Value-stream mapping, also known as material- and information-flow mapping, [1] is a lean [2]-management method for analyzing the current state and designing a future state for the series of events that take a product or service from the beginning of the specific process until it reaches the customer.
The idea behind iterative mapping is to map as short a sequence as possible to ensure unique identification of interaction pairs before reaching the junction site. [ 29 ] [ 30 ] As a result, 25-bp long reads starting from the 5’ end are mapped to the genome at first, and reads that do not uniquely map to a single loci are extended by an ...
Physical map is a technique used in molecular biology to find the order and physical distance between DNA base pairs by DNA markers. [1] It is one of the gene mapping techniques which can determine the sequence of DNA base pairs with high accuracy. Genetic mapping, another approach of gene mapping, can provide markers needed for the physical ...
There are two distinctive mapping approaches used in the field of genome mapping: genetic maps (also known as linkage maps) [7] and physical maps. [3] While both maps are a collection of genetic markers and gene loci, [8] genetic maps' distances are based on the genetic linkage information, while physical maps use actual physical distances usually measured in number of base pairs.
They share the technical paradigm of massive parallel sequencing via spatially separated, clonally amplified DNA templates or single DNA molecules in a flow cell. This design is very different from that of Sanger sequencing —also known as capillary sequencing or first-generation sequencing—which is based on electrophoretic separation of ...
SOLiD applies sequencing by ligation and dual base encoding. The first SOLiD system was launched in 2007, generating reading lengths of 35bp and 3G data per run. After five upgrades, the 5500xl sequencing system was released in 2010, considerably increasing read length to 85bp, improving accuracy up to 99.99% and producing 30G per 7-day run. [10]
There are eight types of maps: Circle Map: used for defining in context; Bubble Map: used for describing with adjectives; Flow Map: used for sequencing and ordering events; Brace Map: used for identifying part/whole relationships; Tree Map: used for classifying or grouping; Double Bubble Map: used for comparing and contrasting