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Protein production is the biotechnological process of generating a specific protein. It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene .
The following is a list of notable proteins that are produced from recombinant DNA, using biomolecular engineering. [1] In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. The prefix "rh" for "recombinant human" appears less and less in the literature.
Sf9 cells, a clonal isolate of Spodoptera frugiperda Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. [1] They can be grown in the absence of serum, and can be cultured attached or in suspension. [2]
Blood, for instance, as of 2012 cannot store high levels of stable recombinant proteins, and biologically active proteins in blood may alter the health of the animals. [16] Expression in the milk of a mammal, such as a cow, sheep, or goat, is a common application, as milk production is plentiful and purification from milk is relatively easy.
In this technology, conventional antibiotic resistance gene is replaced by ccdA in the plasmid while ccdB gene is introduced into the chromosome of the bacteria. This technology allows to remove antibiotic resistance gene but is also able to reach higher yields in recombinant protein production and plasmid DNA. [8]
Biomolecular engineering is the application of engineering principles and practices to the purposeful manipulation of molecules of biological origin. Biomolecular engineers integrate knowledge of biological processes with the core knowledge of chemical engineering in order to focus on molecular level solutions to issues and problems in the life sciences related to the environment, agriculture ...
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