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Molecular breeding is the application of molecular biology tools, often in plant breeding [1] [2] and animal breeding. [3] [4] In the broad sense, molecular breeding can be defined as the use of genetic manipulation performed at the level of DNA to improve traits of interest in plants and animals, and it may also include genetic engineering or gene manipulation, molecular marker-assisted ...
Plant breeding is the science of changing the traits of plants in order to produce desired characteristics. [1] It is used to improve the quality of plant products for use by humans and animals. [2] The goals of plant breeding are to produce crop varieties that boast unique and superior traits for a variety of applications.
Linkage analysis depends upon recent genetic recombination between two different plant lines (as the result of a genetic cross) to identify general regions of interest, with the advantage of requiring few genetic markers to ensure genome wide coverage and high statistical power per allele. Linkage analysis, however, has the disadvantages of low ...
Marker assisted selection or marker aided selection (MAS) is an indirect selection process where a trait of interest is selected based on a marker (morphological, biochemical or DNA/RNA variation) linked to a trait of interest (e.g. productivity, disease resistance, abiotic stress tolerance, and quality), rather than on the trait itself.
For example, DArT has been used to conduct genome-wide analysis among Musa species, which includes bananas and plantains, which led to the development of a phylogenetic cladogram based on genetic markers derived from DArT techniques. These developments enhance breeding knowledge to obtain desirable yields and products.
Gene pyramiding is the simultaneous selection for and/or introduction of multiple genes during plant breeding. [1] Objectives of gene pyramiding includes 1) enhancing trait performance by combining two or more complementary genes, 2) remedying deficits by introgressing genes from other sources, 3) increasing the durability. [2]
DNA shuffling by molecular breeding was first reported in 1994 by Willem P.C. Stemmer. [1] [7] He started by fragmenting the β-lactamase gene that had been amplified with the polymerase chain reaction (PCR) by using DNase I, which randomly cleaves DNA.
Plants in the C22 cycle were 50 cm taller had twice the leaf area index, reached anthesis 7 days later and had a 30% higher harvest index than C0 (Table 1) [clarification needed] Differences in growth were detected early in ontogeny. The root growth of C22 exceeded that of C0 and the ratio of shoot dry mass to root dry mass was reduced by ...