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The purpose of using RNA FISH is to detect target mRNA transcripts in cells, tissue sections, or even whole-mounts. [10] The process is done in 3 main procedures: tissue preparation (pre-hybridization), hybridization, and washing (post-hybridization). The tissue preparation starts by collecting the appropriate tissue sections to perform RNA FISH.
These can be categorized into three groups; cestodes, nematodes and trematodes.Examples include: Acanthocephala; Ascariasis (roundworms); Cestoda (tapeworms) including: Taenia saginata (human beef tapeworm), Taenia solium (human pork tapeworm), Diphyllobothrium latum (fish tapeworm) and Echinococcosis (hydatid tapeworm)
Flow-FISH was first published in 1998 by Rufer et al. [11] as a modification of another technique for analyzing telomere length, Q-FISH, that employs peptide nucleic acid probes [12] of a 3'-CCCTAACCCTAACCCTAA-5' sequence labeled with a fluorescin fluorophore to stain telomeric repeats on prepared metaphase spreads of cells that have been treated with colcemid, hypotonic shock, and fixation to ...
DNA barcoding is a relatively cost-effective and quick method for identifying fish species aquatic environments. [7] Presence or absence of key fish species can be established using eDNA from water samples and spatio-temporal distribution of fish species (e.g. timing and location of spawning) can be studied. [8]
RseqFlow is an RNA-Seq analysis pipeline which offers an express implementation of analysis steps for RNA sequencing datasets. It can perform pre and post mapping quality control (QC) for sequencing data, calculate expression levels for uniquely mapped reads, identify differentially expressed genes, and convert file formats for ease of ...
DNA barcoding is a method of species identification using a short section of DNA from a specific gene or genes. The premise of DNA barcoding is that by comparison with a reference library of such DNA sections (also called "sequences"), an individual sequence can be used to uniquely identify an organism to species, just as a supermarket scanner uses the familiar black stripes of the UPC barcode ...
Quantitative Fluorescent in situ hybridization (Q-FISH) is a cytogenetic technique based on the traditional FISH methodology. In Q-FISH, the technique uses labelled (Cy3 or FITC) synthetic DNA mimics called peptide nucleic acid (PNA) oligonucleotides to quantify target sequences in chromosomal DNA using fluorescent microscopy and analysis software.
Catalog of Fishes is a comprehensive on-line database and reference work on the scientific names of fish species and genera. It is global in its scope and is hosted by the California Academy of Sciences. It has been compiled and is continuously updated by the curator emeritus of the CAS fish collection, William N. Eschmeyer.