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Methods of cell sorting fall into two major categories: fluorescence-activated cell sorting (FACS) and immunomagnetic cell sorting. [2] Due to many years of refinement and increased demand for cell separation however, researchers are working to develop microfluidic sorting devices that have many benefits in comparison to the main types of fluorescence-activated cell sorting and immunomagnetic ...
Timsort is a hybrid, stable sorting algorithm, derived from merge sort and insertion sort, designed to perform well on many kinds of real-world data. It was implemented by Tim Peters in 2002 for use in the Python programming language. The algorithm finds subsequences of the data that are already ordered (runs) and uses them to sort the ...
A bidirectional variant of selection sort (called double selection sort or sometimes cocktail sort due to its similarity to cocktail shaker sort) finds both the minimum and maximum values in the list in every pass. This requires three comparisons per two items (a pair of elements is compared, then the greater is compared to the maximum and the ...
Fluorescence-activated cell sorting (FACS) is a technique for sorting out the cells based on the differences that can be detected by light scatter (e.g. cell size) or fluorescence emission (by penetrated DNA, RNA, proteins or antigens). The system works much like flow cytometry, but will also charge each cell droplet after it has been measured ...
Bubble sort, sometimes referred to as sinking sort, is a simple sorting algorithm that repeatedly steps through the input list element by element, comparing the current element with the one after it, swapping their values if needed. These passes through the list are repeated until no swaps have to be performed during a pass, meaning that the ...
Many single-cell analysis techniques require the isolation of individual cells. Methods currently used for single-cell isolation include: dielectrophoretic digital sorting, enzymatic digestion, FACS, hydrodynamic traps, laser capture microdissection, manual picking, microfluidics, Inkjet Printing (IJP), micromanipulation, serial dilution, and Raman tweezers.
A magnetic-labelled receptor that binds to annexin is added to sperm. Inside normal cells, phosphatidylserine molecules are located within the cell membrane towards the cytoplasm. Nevertheless, in those cells that initiate the apoptotic process phosphatidylserine instead faces the cell membrane outer side, binding to the annexin conjugate.
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