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The majority of tips have a color code for easy spotting like natural (colorless) for low volumes (0.1–10 μL), yellow (10–100 μL), or blue (100–1,000 μL). The corresponding pipette has the same color code, printed on the pipette. For special applications, there are filter-tips available.
Positive displacement pipettes are a type of pipette that operates via piston-driven displacement. [1] [2] Unlike an air displacement pipette, which dispenses liquid using an air cushion in the pipette tip, the piston in a positive displacement pipette makes direct contact with the sample, allowing the aspiration force to remain constant.
Move the pipette to the receiving vessel and dispense the liquid by gently pressing the pipette knob to the first stop. After about one second, keep on pressing pipette knob to the second stop. This action will completely empty the tip from the liquid. Afterwards withdraw the tip from the liquid and release the pipette to the ready position.
As the piston moves upward, driven by the depression of the plunger, a vacuum is created in the space left vacant by the piston. The liquid around the tip moves into this vacuum (along with the air in the tip) and can then be transported and released as necessary. These pipettes are capable of being very precise and accurate.
It was also the first area code assigned in the numbering plan. New Jersey numbering plan areas as shown in the July 1963 NJ Bell telephone directory, when the use of the area code became mandatory. In 1956, New Jersey was divided into two numbering plan areas, a northern area which retained the code 201, and a southern area which received the ...
A small pipette allows for more precise measurement of fluids; a larger pipette can be used to measure volumes when the accuracy of the measurement is less critical. Accordingly, pipettes vary in volume, with most measuring between 0 and 25.0 millilitres (0.00 and 0.88 imp fl oz; 0.00 and 0.85 US fl oz). [2]