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Barfoed's test is a chemical test used for detecting the presence of monosaccharides. It is based on the reduction of copper(II) acetate to copper(I) oxide (Cu 2 O), which forms a brick-red precipitate. [1] [2] RCHO + 2Cu 2+ + 2H 2 O → RCOOH + Cu 2 O↓ + 4H + (Disaccharides may also react, but the reaction is much slower.)
The dye reagent is a stable ready to use product prepared in phosphoric acid. It can remain at room temperature for up to 2 weeks before it starts to degrade. Protein samples usually contain salts, solvents, buffers, preservatives, reducing agents and metal chelating agents.
The [BAr F 4] − anion with four fluorinated aryl groups distributed tetrahedrally about a central boron atom. Tetrakis[3,5-bis(trifluoromethyl)phenyl]borate is an anion with chemical formula [{3,5-(CF 3) 2 C 6 H 3} 4 B] −, which is commonly abbreviated as [BAr F 4] −, indicating the presence of fluorinated aryl (Ar F) groups.
Christen Thomsen Barfoed. Christen Thomsen Barfoed (16 June 1815 – 30 April 1889) was a Danish chemist credited with the development of a method to detect monosaccharide sugars in a solution, now known as the Barfoed's test. [1] [2] Barfoed is also credited with having introduced systematic chemical analyses in Danish agricultural sciences. [3]
Tollens' reagent tests for aldehydes (known as the silver mirror test) The Zeisel determination tests for the presence of esters or ethers; Lucas' reagent is used to distinguish between primary, secondary and tertiary alcohols. The bromine test is used to test for the presence of unsaturation and phenols.
An example of a positive Seliwanoff’s test. Seliwanoff’s test is a chemical test which distinguishes between aldose and ketose sugars.If the sugar contains a ketone group, it is a ketose.
Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper(II) sulfate pentahydrate. [1] It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing substances. [2]
Molisch test (using α-napthol) indicating a positive result (see purple ring). Molisch's test is a sensitive chemical test, named after Austrian botanist Hans Molisch, for the presence of carbohydrates, based on the dehydration of the carbohydrate by sulfuric acid or hydrochloric acid to produce an aldehyde, which condenses with two molecules of a phenol (usually α-naphthol, though other ...