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Phosphate-buffered saline (PBS) is a buffer solution (pH ~ 7.4) commonly used in biological research. It is a water-based salt solution containing disodium hydrogen phosphate, sodium chloride and, in some formulations, potassium chloride and potassium dihydrogen phosphate. The buffer helps to maintain a constant pH.
The majority of biological samples that are used in research are kept in a buffer solution, often phosphate buffered saline (PBS) at pH 7.4. In industry, buffering agents are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics. They are also used in chemical analysis [5] and calibration of pH ...
Hanks' salts is a collective group of salts rich in bicarbonate ions, formulated in 1940 by the microbiologist John H. Hanks. [1] Typically, they are used as a buffer system in cell culture media and aid in maintaining the optimum physiological pH (roughly 7.0–7.4) for cellular growth.
Part of the lysis buffer (usually in a 5% solution in alkaline lysis buffer) in DNA extraction; Reducing surface tension of aqueous solutions during immunostaining (usually at a concentration of 0.1-0.5% in TBS or PBS buffer) Dispersion of carbon materials for soft composite materials; Restricting colony expansion in Aspergillus nidulans in ...
Dulbecco's phosphate-buffered saline, a buffer solution used in biological research DPBS (CONFIG.SYS directive) , a configuration directive in DOS Topics referred to by the same term
BSS (ophthalmic irrigation solution) (produced by Alcon) . Composition per 1 mL: sodium chloride (NaCl) 6.4 mg, potassium chloride (KCl) 0.75 mg, calcium chloride dihydrate (CaCl 2 ·2H 2 O) 0.48 mg, magnesium chloride hexahydrate (MgCl 2 •6H 2 O) 0.3 mg, sodium acetate trihydrate (C 2 H 3 NaO 2 ·3H 2 O) 3.9 mg, sodium citrate dihydrate (C 6 H 5 Na 3 O 7 ·2H 2 O) 1.7 mg, sodium hydroxide ...
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Tris-buffered saline (TBS) is a buffer used in some biochemical techniques to maintain the pH within a relatively narrow range. Tris (with HCl) has a slightly alkaline buffering capacity in the 7–9.2 range. The conjugate acid of Tris has a pK a of 8.07 at 25 °C. The pK a declines approximately 0.03 units per degree Celsius rise in ...