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The rotor can then be fixed within the centrifuge without opening the lid. After the run, the entire rotor assembly, including the lid, is removed from the centrifuge to the hood for further steps, maintaining the samples within a closed system. A ThermoFisher laboratory bench-top centrifuge.
A centrifuge can be a very effective filter that separates contaminants from the main body of fluid. Industrial scale centrifuges are commonly used in manufacturing and waste processing to sediment suspended solids, or to separate immiscible liquids. An example is the cream separator found in dairies.
A cytocentrifuge, sometimes referred to as a cytospin, [1] is a specialized centrifuge used to concentrate cells in fluid specimens onto a microscope slide so that they can be stained and examined. [2] Cytocentrifuges are used in various areas of the clinical laboratory, such as cytopathology, hematology and microbiology, as well as in ...
Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Intact (eukaryotic) cells, macroscopic debris Varies depending on sample Gently lysed cells (e.g. dounce homogenizer) 600 x g 10 min Benchtop fixed-angle centrifuge, or swinging bucket centrifuge Nuclei Cytosol, non-nuclei organelles Supernatant of previous row 15,000 x g 20 min
If we assume a rotor diameter of 20 cm (as in some modern centrifuges [3]), this would correspond to a centripetal acceleration of around 900,000 x g [4] (around 42 times the max speed of a standard, lab benchtop microcentrifuge [5] and between 0.9 to 9 times the max speed of a standard lab ultracentrifuge [6]) or a linear speed of greater than ...
Preparation of microbiological samples in a laminar chamber. A laminar flow cabinet or tissue culture hood is a partially enclosed bench work surface designed to prevent contamination of biological samples, semiconductor wafer, or any particle-sensitive materials.
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