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Plant cryopreservation consist of the storage of seeds, pollen, tissue, or embryos in liquid nitrogen. This method can be used for virtually indefinite storage of material without deterioration over a much greater time-period relative to all other methods of ex situ conservation.
in situ, which consists in managing populations on-site, dynamically evolving in their natural environment. [6] [7] In situ methodologies include: conservation in natural populations (in nature) on farm conservation; ex situ, which consists in conserving individuals or populations out of their natural environments. Ex situ gene bank ...
In-situ conservation is the on-site conservation or the conservation of genetic resources in natural populations of plant or animal species, such as forest genetic resources in natural populations of tree species. [1] This process protects the inhabitants and ensures the sustainability of the environment and ecosystem.
The Centre for Pacific Crops and Trees (CePaCT) plant gene bank in Suva, Fiji, focuses on propagating (and re-propagating) seedlings of plants (using clippings and tissue culture, rather than as seeds), to preserve the genetic diversity of the most important varieties of food crops of the Pacific region, such as banana, taro, breadfruit and yam.
Bioremediation techniques can be classified as (i) in situ techniques, which treat polluted sites directly, vs (ii) ex situ techniques which are applied to excavated materials. [8] In both these approaches, additional nutrients, vitamins, minerals, and pH buffers are added to enhance the growth and metabolism of the microorganisms.
Plant cryopreservation is a genetic resource conservation strategy that allows plant material, such as seeds, pollen, shoot tips or dormant buds to be stored indefinitely in liquid nitrogen. [1] After thawing, these genetic resources can be regenerated into plants and used on the field.
This method can be used for virtually indefinite storage of material without deterioration over a much greater time-period relative to all other methods of ex situ conservation. However, cryo-conservation can be an expensive strategy and requires long term hygienic and economic commitment for germplasms to remain viable.
Cryopreservation is a hallmark method for fungi that do not sporulate (otherwise other preservation methods for spores can be used at lower costs and ease), sporulate but have delicate spores (large or freeze-dry sensitive), are pathogenic (dangerous to keep metabolically active fungus) or are to be used for genetic stocks (ideally to have an ...