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[16] [30] Video-rate imaging not only reduces motion artifacts, but also allows in vivo study of biological processes, even in hand-held mode. It also gives the operator real-time feedback essential for orientation and fast localization of areas of interest. [2] Fig. 3: Five-dimensional imaging of mouse brain perfusion in vivo. (a) Layout of ...
Medical optical imaging is the use of light as an investigational imaging technique for medical applications, pioneered by American Physical Chemist Britton Chance. Examples include optical microscopy , spectroscopy , endoscopy , scanning laser ophthalmoscopy , laser Doppler imaging , optical coherence tomography , and transdermal optical imaging .
The first intravascular in vivo use in a preclinical model was reported in 1994 [6] and first in human, clinical imaging in 2003. [7] The first OCT imaging catheter and system was commercialized by LightLab Imaging, Inc., a company based in Massachusetts formed following a technology transfer in 1997 from Fujimoto's lab (MIT). [8]
Photoacoustic imaging or optoacoustic imaging is a biomedical imaging modality based on the photoacoustic effect.Non-ionizing laser pulses are delivered into biological tissues and part of the energy will be absorbed and converted into heat, leading to transient thermoelastic expansion and thus wideband (i.e. MHz) ultrasonic emission.
Two-photon excitation microscopy of mouse intestine.Red: actin.Green: cell nuclei.Blue: mucus of goblet cells.Obtained at 780 nm using a Ti-sapphire laser.. Two-photon excitation microscopy (TPEF or 2PEF) is a fluorescence imaging technique that is particularly well-suited to image scattering living tissue of up to about one millimeter in thickness.
As stated earlier, the problem is not reluctance by researchers to use in vivo imaging modalities, but rather a lack of suitable ones. For example, although optical imaging provides fast functional data and oxy- and deoxyhemoglobin analysis, [7] it requires a craniotomy and only provides a few hundred micrometres of penetration depth ...