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Stimulated emission was a theoretical discovery by Albert Einstein within the framework of the old quantum theory, wherein the emission is described in terms of photons that are the quanta of the EM field. [5] [6] Stimulated emission can also occur in classical models, without reference to photons or quantum-mechanics.
Stimulated emission depletion (STED) microscopy is one of the techniques that make up super-resolution microscopy. It creates super-resolution images by the selective deactivation of fluorophores , minimizing the area of illumination at the focal point, and thus enhancing the achievable resolution for a given system. [ 1 ]
This technique primarily utilizes fluorophores to visualize the location of the antibodies, while others provoke a color change in the environment containing the antigen of interest or make use of a radioactive label. Immunofluorescent techniques that utilized labelled antibodies was conceptualized in the 1940s by Albert H. Coons. [2] [6] [7]
The beads in SPA are formed from the incorporation of scintillant into small beads known as fluomicrospheres. These are specially designed to bind with specific molecules. When the bead is in close proximity to the radioactive molecule, light is stimulated. The photonmultiplier tube (PMT) can be used to detect the emitted photons.
Schematic diagram of atomic stimulated emission. Stimulated emission (also known as induced emission) is the process by which an electron is induced to jump from a higher energy level to a lower one by the presence of electromagnetic radiation at (or near) the frequency of the transition. From the thermodynamic viewpoint, this process must be ...
Melanin is a chromophore that exists in the human epidermal layer of skin responsible for protection from harmful UV radiation. When melanocytes are stimulated by solar radiation, melanin is produced. [7] Melanin is one of the major absorbers of light in some biological tissue (although its contribution is smaller than other components).
The illumination light is separated from the much weaker emitted fluorescence through the use of a spectral emission filter. Typical components of a fluorescence microscope are a light source ( xenon arc lamp or mercury-vapor lamp are common; more advanced forms are high-power LEDs and lasers ), the excitation filter , the dichroic mirror (or ...
The rate of spontaneous emission (i.e., the radiative rate) can be described by Fermi's golden rule. [17] The rate of emission depends on two factors: an 'atomic part', which describes the internal structure of the light source and a 'field part', which describes the density of electromagnetic modes of the environment.